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A clean water is imperative for the production of high quality food products and an absolutely essentialamphipods_bioassays element to ensure the commercial success of the Galician shellfish. Nowadays, the protection of the coastal marine environment must take into account the legislative framework created by the Water Framework Directive (WFD) (Directive 2000/60/EC), the Marine Strategy Directive (MSD) (Directive 2008/56/EC), and the water quality standards updated by Directive 2013/39/EU.

Ecotox, in collaboration with the Environmental Quality Unit of the ECIMAT, offers services related with Monitoring water quality and chemical and microbial pollution:

 

1. WFD CLASSIFICATION OF ECOLOGICAL STATUS OF WATER BODIES

WFD establishes for the biological indicators 5 categories of quality (high, good, moderate, poor y bad), from lower to higher human alteration of the reference conditions, with their corresponding color code (Table 1).

Table 1
  Sea urchin Amphipods Copepods Bivalves Dafnia Microalgae
Categories PNR Toxicity Mortality(%) Toxicity Mortality(%) Toxicity Mortality(%) Toxicity Mortality(%) Toxicity Mortality(%) Toxicity
HIGH >0.879 Absence                    
GOOD 0.879–0.649 Absence <30 Low <10 Low <20 Absence <10 Low    
MODERATE 0.649–0.508 Low 30-40 Medium 10-30 Medium 20–30 Low 10-30 Medium    
POOR 0.508–0.240 Medium 40-60 High 30-50 High 30-50 Medium 30-50 High    
BAD <0.24 High >60 Very_High >50 Very_High >50 High >50 Very_High    

 

2. A PRIORI RISK ASSESSMENT OF NEW CHEMICALS/MATERIALS, AND DERIVATION OF THEIR WATER QUALITY STANDARDS

According to REACH procedures, the basic aquatic toxicity data-set is conducted with organisms belonging to three trophic levels:

  Trophic level I Trophic level II Trophic level III
Marine Isochrysis spp, Phaeodactylum spp
ISO 10253:2007
Paracentrotus lividus
 ICES TIMES Nº51
Acartia spp
ISO 14669:1999
Cyprinodon variegatus
US-EPA 821-R02-014
Inland Pseudokirchneriella spp, Desmodesmus spp
OECD Prueba Nº201
Acartia spp
ISO 6341:1996
Danio rerio
OECD Prueba Nº236

 

3. BIOASSAYS FOR THE MANAGEMENT OF HARBOUR DREDGING MATERIALS
Management of harbour dredged materials in Spain should include an evaluation of the material’s toxicity using bacterial bioluminescence, sea-urchin and amphipod bioassays according to the guidelines: Directrices para la caracterización del material dragado y su reubicación en aguas del dominio público marítimo-terrestre. COMISIÓN INTERMINISTERIAL DE ESTRATEGIAS MARINAS 2014.

Screening Toxicity Test (TPT)
(with bacteria)
Liquid Phase Bioassay
(with sea urchin)
Solid Phase Bioassay
(with anfipods)
Bioluminiscence test with Vibrio fischeri (MICROTOX)
ISO 11348-3:2007
Fertilization test with Paracentrotus lividus Embryogenesys test with Paracentrotus lividus Survival test with Corophium sp.

 

4. MICROBIOLOGICAL ANALYSIS OF WATERS

Directive 2006/7/EC establishes microbial requirements for transitional and coastal bathing waters on the basis of intestinal enterococci and E. coli (ISO 9308-1:2014).

 

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